A workshop on Toxicology
A workshop on Toxicology

Title: Extraction, quantification, and analysis of pesticide residues (agricultural crops and environmental samples)

Date: 2017-09-03, 8:00-18:30

Capacity: 10

Wide application of pesticides in agricultural systems has resulted in the accumulation of these agrochemicals in crops and the surrounding environment. The presence of pesticides in fruits/vegetables can pose harmful effects on humans and hence, monitoring their residues is seems indispensable. Pesticide residue analysis at low concentrations in different samples is a complex procedure which requires adopting suitable, highly efficient, and well-validated extraction and quantification methods. Up to now, a vast number of extraction and measurements methods have been developed and utilized. However, choosing the best method for a special analysis has always been an issue among the researchers and requires high knowledge about these approaches.

In this workshop, the attendees will be provided by comprehensive information on pesticide residue analysis approaches which includes sampling, extraction, clean-up, quantitation, and validation methods. In the experimental session, a widely adopted method (QuEChERS) will be used to extract pesticide residues from crop and soil samples and the residues will be measured using high-performance liquid chromatography (HPLC). Furthermore, validations tests will be performed to validate the extraction and measurement methods.

Theory session

  • Sampling methods and experimental design in pesticide residue studies.
  • Sample preparation, extraction, and clean-up methods with a focus on newly developed approaches.
  • Quantification of pesticides using chromatographic approaches (gas and liquid chromatography).
  • Method validation (recovery, calibration and linearity, detection limit, etc.)

Experimental session

  • Extraction of pesticides from fruit and soil samples using QuEChERS method.
  • Quantification of pesticide residues using high-performance liquid chromatography (HPLC).
  • Estimating various validation criteria (recovery, calibration and linearity, detection limit) for the extraction and quantification methods.

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